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In Vitro Fertilization (IVF) Process

Sperm Analysis

This is a test to assess the quantity, quality, and morphology of sperm in order to analyze the causes of male infertility. The test is divided into two parts: macroscopic examination and microscopic examination.

Macroscopic Examination

  • Volume: refers to the amount of semen collected (it should be no less than 1.5 milliliters).
  • pH: Semen should be slightly alkaline (the pH value should be greater than 7.2).
  • Viscosity: This affects sperm motility (when semen is dropped, it should not extend more than 2 centimeters).
  • Liquefaction: Semen typically liquefies within 1 hour.

Microscopic Examination

1. Sperm Count or Concentration

  • This refers to the total number of sperm cells present (it should be at least 15 million sperm per milliliter).

2. Sperm Motility

  • This refers to the percentage of sperm that are still moving (at least 40% should be motile).
  • The sperm can be graded as follows:
    • •Grade A – Sperm that moves quickly in a straight line forward.
    • •Grade B – Sperm that are still moving forward but in a curved or circular motion.
    • •Grade C – Sperm that do not move forward but show some movement, such as tail or body movement.
    • •Grade D – Sperm that shows no movement at all.
    • (At least 32% of sperm should be Grade A.)

3. Sperm Morphology

  • This refers to the examination of the shape of sperm cells. Abnormalities in sperm shape can be classified into abnormalities in the head and abnormalities in the tail. (Sperm should have a normal morphology rate of at least 4%).

** Reference values are based on the WHO 2010 5th Edition standards. **

Tips for Preparing (Male)

  • Please make an appointment at the EKI-IVF Clinic for sperm quality analysis (Sperm Analysis).
  • A blood test for screening will be performed prior to sperm collection.
  • Sperm collection will be done through masturbation in a private room provided for this purpose.
  • After sperm collection, the sample will be sent directly to the Andrology Lab for sperm analysis.
  • The results of the sperm analysis will be available in approximately 1-2 hours.